Coding

Part:BBa_K3349003:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)


Pectin Lyase Pnl-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli. The his tag was placed on the C-terminus. A TEV cut site was added between the GFP and the Pectinase in case cleavage is needed after purification.

Source

The GFP protein was taken from the uniprot database (P42212). The pectin lyase was also taken from the uniprot database and the sequence ID from NCBI is KAA8782864.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.